Enhancing UDP-rhamnose supply for rhamnosylation of flavonoids in Escherichia coli by regulating the modular pathway and improving NADPH availability

In this study, the recombinant strain with the highest UDP_x0002_rhamnose supply was reconstructed by comparing UDPglucose biosynthesis pathways, enhancing the UTP biosynthesis pathway, and overexpressing NADPH regenerator. The efficient synthesis pathway described in this study for UDPrhamnose biosynthesis could be widely used for rhamnosylation flavonoids in E. coli.

搜集汇总

背景与挑战

背景概述

该数据集研究通过比较UDP-葡萄糖生物合成途径、增强UTP生物合成以及过表达NADPH再生器，重构了大肠杆菌重组菌株，以最大化UDP-鼠李糖的供应。所描述的UDP-鼠李糖高效合成途径可广泛应用于大肠杆菌中黄酮类化合物的鼠李糖基化修饰。