Kinetic parameters of PcGSTFuAs and CcGSTFuAs towards MUA in absence or presence of GSH.
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The apparent Km values were determined using a concentration range of 100–800 µM in absence (−GSH) or presence of 5 mM GSH (+ GSH). The apparent Km and kcat values were calculated by nonlinear regression using the Michaelis-Menten equation (r2>0.99). Data are represented as mean ± S.D. (n±3). ND: Not Detected. The detection limit was estimated at 0.5 mUI.
创建时间:
2015-12-02



