Next Generation Sequencing Facilitates Quantitative Analysis of Wild Type (WT) and dek41 Transcriptomes

Analysis of gene expression level. The hypothesis tested in the present study was that dek41 mutation affects cis-splicing of nad4 intron 3 in mitochondria in mutant kernels. Results provide important information of the gene expression level of mitochondrial membrane and metabolic process pathway associated genes. Overall design: Endosperm mRNA profiles of 12 DAP wild type and dek41 mutant were generated by deep sequencing, using Illumina Hiseq 4000 (Zea mays).