Initial single 2fold probe was designed at the Landegren lab in Uppsala University, and the following experiments were performed at the Broad Institute at the Mikkelsen lab. 12000 2fold probes were sy
Numerous real-time PCR devices and master mixes are available on the market. To perform reliable high-quality data, PCR master mix, and equipment need to be optimal. However, general lab optimized pro
The SIBA reaction was performed with either a fully homologous target template (SB-template) or with templates containing 1–4 base point mutation(s). The results are expressed as the delay in the thre
