Phenotyping of CD1c+ isolated PBMCs
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To phenotype cells that are isolated when applying the "CD1c+ DC isolation kit" to PBMCs. We used CD45, HLA-DR, CD11c, CD14, SIRPa and CD1c Conclusion: The CD1c+ fraction contains CD11c+CD14-SIRPa+ DCs, CD14+SIRPa+ monocytes as well as a minor fraction of DR-CD11c- cells (presumably T cells according to our other experiments). As seen in earlier work, CD1a and CD103 are not expressed by blood MNPs. Cytometer performance and tracking was performed the same day. Beads were used for compensation (OneComp eBeads, eBioscience)
创建时间：
2016-06-01
