Vittecoq-et-al_Evolutionary-Biology

1. Ant behavioural reaction to stimuli presented at the entrance of the domatium for 3 minutes. 2. Ant behavioural reaction to leaflet damage and pure compounds, using the paired-leaves protocol. 3. Quantification of leaf compounds. Leaflets of three taxa of Leonardoxa africana (subspp. africana, letouzeyi and gracilicaulis) were soaked in hexane for 30 minutes. 2.9 µg of nonane was added to each sample as an internal standard. Amounts of (E)-2-hexenal and methyl salicylate were determined by gas chromatography / mass spectroscopy. 4. Antennal responses of ants (measured by electroantennography) to compounds. 5. Antennal responses of ants (measured by electroantennography) to increasing amounts of compounds.