Cultivated Soybean RNA-Seq of salt treatment

To obtain a comprehensive understanding of transcriptomic reprogramming under salt stress, we performed whole genome RNA sequencing on salt-treatment soybean seedlings, on two tissues in a time-course experiment (0h, 1h, 2h, 4 h, 24h and 48h). This time series dataset enables us to identify important hubs and connection of gene expressions. We highlighted the analysis of phytohormone signaling pathways and their possible cross-talking. Gene expression regulation also controls adjustment of carbon and nitrogen metabolism. In general, the treated seedlings had turned off the photosynthetic mechanism and enhanced sugar catabolism to provide energy for survival. Primary nitrogen assimilation was shut down while re-distribution of nitrogen resources was activated. Genes for other protective mechanisms were also induced, including structural modification, ion-sequestering, and scavenging of reactive oxygen species.

为全面解析盐胁迫（salt stress）下的转录组重编程（transcriptomic reprogramming），本研究针对盐处理大豆幼苗的两类组织，开展了时间梯度实验（time-course experiment），设置0h、1h、2h、4h、24h及48h共6个时间点，进行全基因组RNA测序（whole genome RNA sequencing）。该时间序列数据集可用于鉴定基因表达的关键枢纽节点与调控关联。本研究重点分析了植物激素信号通路（phytohormone signaling pathways）及其潜在的交叉互作机制。基因表达调控同时参与碳代谢（carbon metabolism）与氮代谢（nitrogen metabolism）的稳态调节。总体而言，盐处理的大豆幼苗会关闭光合机制（photosynthetic mechanism），并增强糖分解代谢（sugar catabolism）以提供生存所需能量。初级氮同化（primary nitrogen assimilation）过程被抑制，而氮资源的重新分配通路被激活。其余保护相关机制的基因亦被诱导表达，涵盖结构修饰、离子螯合及活性氧（reactive oxygen species）清除等通路。

搜集汇总

背景与挑战

背景概述

该数据集是一个关于盐处理栽培大豆的RNA-Seq研究，通过全基因组测序分析盐胁迫下大豆幼苗的转录组动态变化，涵盖两个组织和多个时间点（0h至48h），旨在揭示基因表达调控、植物激素信号通路和碳氮代谢适应机制。数据集包含36个原始序列读数样本，由香港中文大学组织，于2018年更新，具有农业研究价值。