Here, we investigate how delays in plasma processing affect peptide-centric “bottom-up” proteomics, using multiple reaction monitoring mass spectrometry (MRM-MS). We used validated Tier 2-level assay
A proper internal standard choice is critical for accurate, precise, and reproducible mass spectrometry-based proteomics assays. Synthetic isotopically labeled (SIL) proteins are currently considered
Methionine-containing peptide fragments detected that show a mass increase of 16 or 32 Da after proteolytic digestion of TmPOx (inactivated during turnover of 100 mM D-glucose, treated with endogenous
