cAMP-dependent protein kinase involves calcium tolerance through the regulation of Prz1 in <i>Schizosaccharomyces pombe</i>
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The cAMP-dependent protein kinase Pka1 is known as a regulator of glycogenesis, meiosis, and stress responses in <i>Schizosaccharomyces pombe</i>. We demonstrated that Pka1 is responsible for calcium tolerance. Loss of functional components of the PKA pathway such as Git3, Gpa2, Cyr1, and Pka1 yields a CaCl<sub>2</sub>-sensitive phenotype, while loss of Cgs1, a regulatory subunit of PKA, results in CaCl<sub>2</sub> tolerance. Cytoplasmic distribution of Cgs1 and Pka1 is increased by the addition of CaCl<sub>2</sub>, suggesting that CaCl<sub>2</sub> induces dissociation of Cgs1 and Pka1. The expression of Prz1, a transcriptional regulator in calcium homeostasis, is elevated in a <i>pka1∆</i> strain and in a wild type strain under glucose-limited conditions. Accordingly, higher expression of Prz1 in the wild type strain results in a CaCl<sub>2</sub>-sensitive phenotype. These findings suggest that Pka1 is essential for tolerance to exogenous CaCl<sub>2</sub>, probably because the expression level of Prz1 needs to be properly regulated by Pka1.
