Effect of endophilin A deficiency in mouse hippocampus

We tested how complete or partial loss of endophilin A1, A2 and A3 affects gene expression in mouse hippocampus. Total loss of endophilin (triple knock-outs, TKO) was assessed in newborn mice, since the TKO mice only survive only several hours after birth. Partial loss of endophilin (endoA1,A2 double knock-out, DKO) was assessed between 2-3 weeks of age (p13-21).本研究探究了内吞蛋白（endophilin）A1、A2与A3完全或部分缺失对小鼠海马体基因表达的影响。针对内吞蛋白完全缺失模型，即三重基因敲除（triple knock-outs, TKO），本研究于新生小鼠中开展检测；由于该TKO模型小鼠仅能在出生后存活数小时，因此选择新生阶段进行实验。针对内吞蛋白部分缺失模型，即A1、A2双基因敲除（double knock-out, DKO），本研究则在小鼠2至3周龄（出生后第13至21天，p13-21）阶段开展相关检测。