Homo sapiens Genome sequencing. Homo sapiens

Recently, base editors, composed of cytidine deaminase, Cas9 nickase (nCas9), and uracil DNA glycosylase inhibitor (UGI), have recently been developed to substitute a C at a target site with T without generating DSBs in plant, yeast, mouse zygotes, and human cells, and shown to be >100-fold more efficient than HR at generating point mutations. Moreover, the genome-targeting scope has been increased by using staphylococcus aureus CRISPR/Cas9 (SaCas9) with modified protospacer adjacent motif (PAM) recognition.