The post-transcriptional addition of nucleotides to the 3´ end of RNA regulates the maturation, function, and stability of RNA species in all domains of life. Here, we show that, in flies, 3´ terminal
RIN values were measured using the Eukaryote Total RNA Pico assay on the Agilent 2100 Bioanalyzer (Agilent Technologies). RNA yield is based on a sample eluate of 35 µ L. RNA concentration was measure
The use of low quality RNA samples in whole-genome gene expression profiling remains controversial. It is unclear if transcript degradation in low quality RNA samples occurs uniformly, in which case t
Plants generally possess a strong ability to regenerate organs; for example, in tissue culture, shoots can regenerate from callus, a clump of actively proliferating, undifferentiated cells. Processing
The total amount of RNA extracted from each sample and two quality measures are shown for each chick, eye, tissue and ZT sampling time. chick ID, an arbitrary identification number used for this exper
