Paternal cold exposure programs offspring brown adipose tissue function

Strategies to induce brown adipose tissue mass and functionality have been the focus of intense research in the last years to identify new approaches to treat obesity and its associated co-morbidities. In a mouse model system we demonstrate that pre-conception cold exposure leads to improved systemic metabolism and protects from diet induced obesity, exclusively through sperm via the paternal lineage. To further identify the mechanism, we did RNA sequencing analysis. Overall design: C57BL/6 mice (7-week of age) from normal mice (Ctrl) or paternal cold exposed (7 days before mating) mice (PCE) were housed at room temperature (RT) or cold induced for 3 days (3d CE). Four groups (n=3) are named as “Ctrl-RT”, “Ctrl-3d CE”, “PCE-RT” and “PCE-3d CE”.

搜集汇总

背景与挑战

背景概述

该数据集包含24个来自Illumina HiSeq 4000平台的RNA测序样本，用于研究父代冷暴露对后代棕色脂肪组织功能的编程作用。数据均为单端测序，旨在揭示父代环境因素通过表观遗传途径影响后代代谢适应性的分子机制。