RNA-seq analysis of gene expression regulated by LSD1 in CD8+ TILs

T cell dysfunction is a hallmark of many cancers. Features of dysfunctional T cells and mechanisms leading to T cell dysfunction remain incompletely understood. Significant chromatin changes happen when T cells become dysfunctional, implicating a critical role of epigenetic regulators in such process. Here we study the role of LSD1 in regulating T cell function in cancer and perform a RNA-seq analysis of gene expression program controlled by LSD1. MC38 tumors were implanted to Cd4-Cre+Lsd1f/f mice and littermate control for 14 days. Tumors were harvested and tumor-infiltrating lymphocytes were enriched, then subjected to CD8+ T cells isolation by microbeads and positive selection. Afterwards, CD8+CD44+PD1+ cells were sorted by FACS. Total RNA was extracted and subjected to ribo-RNA depletion, before used for making library. The prepared libraries were sequenced at 75bp from both ends.