To study the microbial community composition of Xiaoqu, as a starter for Chinese chixiang-flavor liquor fermentation , ITS sequencing was applied for fungal community of mature Xiaoqu.
New PCR primers were designed with the following objectives: 1) targetting the ITS2 region to minimize bias due to introns at the 3' end of the SSU, 2) have the broadest possible coverage of Fungi, 3)