RNA-seq analysis for pointed tip fruit in tomato

To gain insight into the mechanism of PT in controlling the development of fruit with pointed tips, we conducted RNA sequencing (RNA-seq) in the distal end of the fruit from CR-ptH and wild-type TS-9. Fruits were harvested at 5 d post anthesis (DPA) from CR-ptH lines and wild-type TS-9. Pointed tips (CR-ptH) and the corresponding pericarp (TS-9) were collected from the distal end of the fruit. Samples were collected with three biological replicates and sent to Seqhealth Technology (Wuhan, China) for RNA-seq analysis.