IRF4-dependent division-coupled reprogramming events [IRF4cKO.ATACseq]

To assess the timing and scope of IRF4–dependent reprogramming in vivo, CTV-labeled IRF4–sufficient and –deficient B cells were transferred into mMT hosts. One day later, hosts were challenged with 50 mg LPS. Three days post-LPS challenge, transferred cells were recovered and sorted from divisions 0, 1, 3, 4, 5, and 6 as determined by CTV dilution for ATAC–seq. ATAC-seq on control and IRF4-deficient B cells at discrete divisions