Transcriptional reprogramming of skeletal muscle during <i>T</i>. <i>spiralis</i> infection in Rag1^-/- mice.

Microarray-based gene expression profiling of diaphragms of uninfected and infected Rag1-/- mice on 0, 2 and 7 post IV infection. (A) Hierarchical clustering and heatmap representation of 1529 genes differentially regulated during a time course of T. spirals muscle infection (2-fold, FDR ≤ 0.05). Four clusters of coordinately regulated genes are indicated. Color pattern represents row Z-score. (B) Bubble chart showing results of Gene Ontology (GO) enrichment analysis. Bubble size indicates number of genes associated with each term. Bubble color indicates whether genes associated with each term were upregulated (red) or downregulated (green), while color intensity indicates fold enrichment. (C) GSEA enrichment plot for four selected pathways with normalized enrichment scores (NES) and false discover rates (FDR). Collagen formation, TCA cycle/electron trans. and glycolysis: 7dpi vs naïve. Interferon signaling: 2dpi vs naïve. (D) Six selected genes from each GSEA signature panel (C). Maximum mean fold change (FC) between naïve and infected Rag1-/- mice is shown.