RNA-seq profiling of gene expression in Ezh2-deficient and control murine lung epithelial and stromal cell populations at day E16.5

Epithelial (CD31-CD45-EpCAM+) and stromal (CD31-CD45-EpCAM-) lung cells were derived by FACS from ShhCre;Ezh2fl/fl and control ShhCre;Ezh2fl/+ mouse embryos at day E16.5 (3 biological replicates per genotype-tissue combination). Total RNA extracted from the samples was subjected to NGS library preparation using TruSeq Stranded Total RNA with Ribo-Zero (Illumina). Completed libraries from different samples were sequenced on HiSeq 2000 TruSeq with SBS Kit v3 - HS reagents (Illumina) as 100 bp single end reads at the Australian Genome Research Facility. RNA-seq gene expression profiles from Ezh2 deficient and control lung epithelial and stromal cell populations at day E16.5 (3 replicates per genotype-tissue combination).肺上皮细胞（CD31⁻CD45⁻EpCAM⁺）与肺间质细胞（CD31⁻CD45⁻EpCAM⁻）通过荧光激活细胞分选术（Fluorescence-Activated Cell Sorting，FACS），从胚胎期16.5天（E16.5）的ShhCre;Ezh2fl/fl及对照ShhCre;Ezh2fl/+小鼠胚胎中分离得到，每组基因型-组织组合设置3个生物学重复。提取上述样本的总RNA，采用Illumina公司的TruSeq Stranded Total RNA with Ribo-Zero试剂盒进行下一代测序（Next-Generation Sequencing，NGS）文库构建。不同样本的构建完成文库于澳大利亚基因组研究中心，采用Illumina公司的HiSeq 2000 TruSeq测序系统与边合成边测序（Sequencing-by-Synthesis，SBS）试剂盒v3-HS试剂进行测序，测序模式为100碱基单端读段。本数据集包含胚胎期16.5天Ezh2缺陷型与对照型肺上皮细胞、肺间质细胞群体的RNA测序（RNA Sequencing，RNA-seq）基因表达谱，每组基因型-组织组合设置3个生物学重复。