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The objectives are to estimate biases associated with the DNA purification of soils and evaluate the effect of a DNA preservation solution in the composition of the microbial community. To do this, DNA extractions efficiencies were compared among single strains, a mix of strains and soil microbial communities. The 16S ribosomal RNA gene of soils was amplified and sequenced to compare structural differences of microbial communities resulted from two commercial DNA purification kits. Besides, soil samples were collected from the Delta Nigeria and transported to the Netherlands using a DNA preservation solution to evaluate the stabilization of the DNA microbial community.

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2020-09-02
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