Single cell RNA-seq analysis for normal lymphocytes and IRF4-driven zebrafish tumors

Single cell RNA-seq analysis was carried out for 1 normal sample from a control zebrafish line (lck-mCherry) and 5 non-tumor and 14 tumor samples from the IRF4 transgenic line (lck-IRF4). Tumor cells were harvested from the animals with or without heterozygous p53 mutation at different time points of tumor development. Human IRF4 gene was overexpressed together with mCherry under the zebrafish lck promoter. Samples were harvested from control or IRF4-transgenic zebrafish (lck-IRF4) with or without heterozygous p53 mutation. mCherry positive cells sorted by flow cytometry. Normal cells was prepared from a control zebrafish which overexpresses only mCherry. Single cell RNA-seq analysis was performed by 10X Genomics using 3' Chromium Single Cell Technique.