Inference of differentiation time for single cell transcriptomes using cell population reference data. Mus musculus

To understand the molecular mechanisms of neural commitment from mouse ESCs, we selected very dense time points during neural differentiation based on qPCR results and performed mRNA-sequencing at both of cell population and single cell levels. Overall design: Two neural induction protocols were used. 21 time points were selected for cell population mRNA-seq. Of them, 8 time points were selected for single cell RNA-seq, and for each time point, 8 individual cells were selected.