Additional file 4: of Lineage-specific duplication of amphioxus retinoic acid degrading enzymes (CYP26) resulted in sub-functionalization of patterning and homeostatic roles

Conserved retinoic acid response elements (RAREs) found in the Branchiostoma lanceolatum, Branchiostoma belcheri, and Branchiostoma floridae CYP26 clusters. B. lanceolatum RAREs recognized and bound by the B. lanceolatum RAR/RXR heterodimer in vitro (Fig. 8) are indicated in black, RAREs that do not associate with the B. lanceolatum RAR/RXR heterodimer in vitro are shown in grey. The in vitro validated B. lanceolatum RAREs composed of two direct repeats (DRs) and separated by a 5 nucleotide spacer (i.e. the validated B. lanceolatum DR5-type RAREs) were used to calculate a consensus B. lanceolatum DR5 RARE by multiple expectation maximization algorithm for motif elicitation (MEME) analysis. (XLSX 77 kb)