Transcriptomic analysis in airway epithelial cells in HDM-sensitized and SCD1 inhibitor-treated or vehicle-treated mice

Purpose: To elucidate the effects of SCD1 inhibitor in allergic airway inflammation. Method: Wild-type mice were sensitized intratracheally twice with house dust mite (HDM) extract at a 7-day interval. Along with that, an SCD-1 inhibitor, A939572 was also administered intraperitoneally daily. Airway epithelial cells were isolated by a cell sorter 48 h after the last senstization. N=2 each. Results: Using an optimized data analysis workflow, 385 transcripts showed differential expression between SCD1 inhibitor-treated mice and DMSO (vehicle)-treated mice with adjusted p value <0.05. Overall design: mRNA profiles of airway epithelial cells isolated from the lung of SCD1 inhibitor-treated mice and DMSO-treated mice after HDM sensitization.