Single cell sequencing of e13 Embryonic Thymuses

Purpose: To identify and compare all the cell subsets present in Normal and 22q11.2 DS Hypoplastic thymus We have identified 17 distinct cell clusters in normal e13 embryonic thymus. While both normal and 22q11.2 DS Hypoplastic thymus have same number of cell clusters, the transcriptome pattern varies significantly between them. Our scRNA sequencing results provide mesenchymal cells driven thymus hypoplasticity in 22q11.2 DS thymus. scRNA seq is performed using e13 embryonic thymuses from control, Tbx1neo2/neo2 and Foxn11089/1089 thymuses研究目的：鉴定并比较正常胸腺与22q11.2缺失综合征（22q11.2 DS）发育不全胸腺中的所有细胞亚群。本研究在正常胚胎第13天（embryonic day 13, e13）胸腺中鉴定出17个独立的细胞簇。尽管正常组与22q11.2 DS发育不全胸腺的细胞簇总数一致，但二者的转录组模式存在显著差异。本研究的单细胞RNA测序（single-cell RNA sequencing, scRNA-seq）结果揭示了22q11.2 DS胸腺中由间充质细胞驱动的胸腺发育不全机制。本次单细胞RNA测序的样本来源于对照组、Tbx1neo2/neo2及Foxn11089/1089基因型的胚胎第13天胸腺组织。