unigene.fasta.SSR_primer.xls
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The searching principle was that only di-, tri-, tetra-, penta- and hexa-nucleotides with a minimum of 8, 5, 5,4 and 3 repeats will be considered as SSR loci. The primers of each EST-SSRs were designed using Primer Premier 5.0 (Premier Biosoft International, CA, USA) with following criterias: PCR fragment size- 100-350 bp; primer length- 16-24 bp; Tm- 55-65 °C; GC content- 40-60 %.
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figshare创建时间：
2017-09-07
