CD34 derived cultured human megakaryocytes were treated with interferon alpha (1000U/ml) for 8 hours and RNASeq of CD61 purified MKs were performed. PBS treated megakaryocytes were used as a control.
High-througput sequence was demonstrated with NovaSeq 6000 (Illumina). First, extracted RNAs were purified by poly(A) capture. Resultant mRNAs were then fragmented and reverse-transcribed into single-
