Role of Tudor-SN in tick dsRNA-mediated RNAi.

Ticks were injected with dsRNA targeting Tudor-SN (aTudor or bSN domain) and SUB to see the effect of Tudor-SN gene knockdown on SUB expression. Gene knockdown was evaluated with respect to Rs86 control. Statistical analysis was conducted with normalized Ct values between test and control groups by Students t-test. Only significant values (P<0.05) are shown. Two independent experiments with 20 ticks each were conducted. Values are shown as Ave±SD (number of independent experimental replicates (N) = 20). Abbreviation: NS, not significant. Role of Tudor-SN in tick dsRNA-mediated RNAi.