CD4+ T cell memory counts and representative flow cytometry staining.

(A–C) The absolute number of CD4+ T cell subsets in VNPs and PPs was measured by flow cytometry and lymphocyte count by complete blood cell (CBC) counts. (A) The absolute number of CD4+ TEM cells; (B) The absolute number of CD4+ TCM cells; (C) The absolute number of CD4+ TSCM cells in VNPs and PPs. (D) Representative flow cytometry staining for each CD4+ T cell memory subset. p values from Mann Whitney T test, line reflects median. Circles, VNPs; Squares, PPs.