Data from the manuscript: Ishii N, Suga Y, Hagiya A, et al. Dynamic simulation of an in vitro multi-enzyme system. FEBS Lett. 2007;581(3):413-420. doi:10.1016/j.febslet.2006.12.049
Kinetic constants for the hydrolysis of pNPP are included for comparison. Kinetic constants for the hydrolysis of two periplasmic TpbB peptide substrates by TpbA.
The source datasets are offered in the form of Excel file for all the plots in the main text and supplementary information. The MATLAB code for image processing in the manuscript is also offered.
aWhen calculating the Km for isocitrate, the concentration of D-isocitrate was calculated as 50% of the total DL-isocitrate in this study. “na” indicates no measurable activity. Comparison of the kine
