Sensitivity and specificity of gene methylation at optimal cut-off values for detection of colon cancer tissue.

TaqMeth V is expressed as mean±SD, and TaqMeth V is described in Materials and Methods. P value was derived from Wilcoxon matched-pairs signed-ranks test. aPT vs. PN. *P<0.01 was considered significant. P value was derived from the Wilcoxon-Mann-Whitney test. bPT vs. NN. AUROC is expressed as mean±SD, and optimal cut-off values were calculated from ROC analysis. cP value in ROC analysis (PT vs. NN). dP value in Fisher's exact test (PT vs. NN). Sensitivity, positive methylation/total tumor cases (PT); Specificity, negative methylation/total normal cases (NN). % Methylation in PN was based on cut-offs from PT vs. NN. Methylation level below the cut-offs was considered as unmethylated and over the cut-offs were as methylated. PN, corresponding normal colon mucosa from CRC patients; PT, CRC tissues; NN, colon normal epithelium from non-cancer patients.