TRACKING ATP-DEPENDENT PROTEIN DYNAMICS

Adenosine triphosphate (ATP) drives many critical cellular processes. We will use laser-induced release from a caged, inactive form of ATP followed by short X-ray pulses at the dedicated time-resolved X-ray solution scattering (TR-XSS) beamline ID09 at ESRF-EBS to study protein conformational change in real time. We aim to track domain movements involved in regulation of membrane protein transporters critical to human health. The pump-and-probe methodology will determine: 1.) kinetics and structural dynamics of a human Ca2+ ATPase that regulates beating of the heart; 2.) lipid regulatory effects on domain movements in Ca2+ transport; 3.) structural dynamics of regulatory internal domains in Cu+ transport. The results will provide better understanding of membrane transport regulation and cooperative dynamics in biologically important proteins, and significantly increase the number of protein targets for similar time-resolved X-ray studies.三磷酸腺苷（ATP）介导诸多关键细胞生命过程。本研究将依托欧洲同步辐射装置-欧洲光源（ESRF-EBS）专用时间分辨X射线溶液散射（TR-XSS）光束线ID09，通过激光诱导释放笼状非活性形式的ATP，并结合该光束线的短X射线脉冲，实时解析蛋白质的构象变化。我们的研究目标为追踪与人类健康密切相关的关键膜蛋白转运体的结构域运动。本研究采用泵浦-探测方法学，旨在完成三项研究内容：1. 调控心脏搏动的人类钙ATP酶的动力学与结构动力学过程；2. 脂质对钙转运过程中结构域运动的调控效应；3. 铜离子转运过程中调控性内部结构域的结构动力学。本研究成果将深化对生物重要蛋白质中膜转运调控机制与协同动力学的理解，同时显著扩充可用于此类时间分辨X射线研究的蛋白质靶标数量。

搜集汇总

背景与挑战

背景概述

该数据集来自欧洲同步辐射设施（ESRF），专注于使用时间分辨X射线溶液散射（TR-XSS）技术实时研究ATP依赖的蛋白质动态。它旨在跟踪膜蛋白转运体（如人类Ca2+ ATPase和Cu+转运蛋白）的构象变化和调控机制，以增进对生物重要蛋白质协同动态的理解。数据集包括实验数据、报告和元数据，发布于2025年，采用Creative Commons Attribution 4.0许可证公开访问。