Identification of DDX5 target genes in spermatogonia. Identification of DDX5 target genes in spermatogonia

Sustained spermatogenesis in adult males and recovery of fertility following germ cell depletion are dependent on undifferentiated spermatogonia with self-renewal potential. Self-renewal of undifferentiated spermatogonia is dependent on a complex network of transcriptional and post-transcriptional regulatory factors. DDX5 is known to act as a transcriptional co-regulator in other systems through interactions with key transcription factors. Here, we performed ChIP-Seq to identify target genes of DDX5 in cultured undifferentiated spermatogonia in order to elucidate its role in maintenance of the male germline. Overall design: Chromatin immunoprecipitations using anti-DDX5 were performed using digested chromatin from two independently derived cultured undifferentiated spermatogonia from CBA mice.