Cleavable Hydrophobic Derivatization Strategy for Enrichment and Identification of Phosphorylated

Herein, a cleavable hydrophobic derivatization (CHD) strategy was established for the enrichment and identification of pLys peptides. By CHD, 2,5-dioxopyrrolidin-1-yl-3-(decyldisulfanyl)propanoate (Dec-disulf-NHS) was synthesized to react with lysine dephosphorylated peptides, and then the derived peptides were captured by a C18 column, followed by the cleavage of hydrophobic chain to leave the specific label on the target peptides.