Dataset for: ZNF384-fusion proteins have high affinity for the transcriptional co-activator EP300 and aberrant transcriptional activities

ZNF384 fusion (Z-fusion) genes have recently been identified as recurrent fusion genes of B-cell precursor acute lymphoblastic leukemia (BCP-ALL) and have been detected in 7-17% of Philadelphia chromosome-negative BCP-ALL cases. We selected SALL4 and ID2 as potential Z-fusion-specific transcriptional targets that might lead to differentiation disorder of Z-fusion-positive ALL. The introduction of EP300-ZNF384 and SYNRG-ZNF384 induced the expression of these genes. Z-fusion proteins exhibited stronger transcriptional activities on the promoter or enhancer region of these genes than Wild-Z. Furthermore, GST pull-down assay revealed that Z-fusion proteins associated more strongly with EP300 than Wild-Z. Co-expression of EP300 specifically enhanced the transcriptional activities of Z-fusion proteins. We propose the increased EP300-binding of Z-fusion proteins as a mechanism for their increased transcriptional activities.