Gene expression profiling in lungs of mice with mesenchyme-specific GR ablation (GRCol1-Cre)

The goal of the experiment was to assay the role of the glucocorticoid receptor (GR) in development of mesenchynmal cells of the lung occuring between the 16 and 18 day of embryonal development. Two time points, E16.5 and E18.5, were analyzed for mutants (GRCol1-Cre) and controls, respectively. For each of the four conditions, five GeneChips were used each representing one animal.本实验旨在探究糖皮质激素受体（glucocorticoid receptor, GR）在肺间充质细胞发育过程中的作用，该发育过程发生于胚胎发育第16至18天区间内。实验针对突变体（GRCol1-Cre）与对照组，分别以E16.5和E18.5两个时间点开展分析。四种实验条件下，各使用五张基因芯片（GeneChips），每张芯片对应一只实验动物。