Stroma extracts were isolated from 2-week-old WT plants and incubated with either PUMPKIN-specific antibodies or with the pre-immune serum. IgGs were captured with SiMAG-Protein G beads (Chemicell) an
MicroRNA (miRNA)-guided target RNA expression is vital for a wide variety of biological processes in eukaryotes. The integration of miRNAs in diverse biological networks relies upon the confirmation o
High-throughput sequencing of RNAs isolated by cross-linking immunoprecipitation in Metarhizium robertsii Overall design: we isolated and identified small RNAs including miRNAs and their targets assoc