FUS-CHOP and ISWI chromatin remodeling in human myxoid liposarcoma

We identified novel protein-protein interactions between FUS-CHOP, a fusion oncoprotein that drives myxoid liposarcoma, and SNF2H, the ATPase subunit of the imitation switch (ISWI) chromatin remodeling complex. We used antibodies for FUS-CHOP, SNF2H, and H3K27ac to profile localization of these proteins and histones marks on chromatin in human MLPS cell lines and show that colocalization of FUS-CHOP and SNF2H occurs at new enhancers marked by H3K27ac. ChIP-seq and CUT&RUN performed using antibodies for FUS-CHOP, H3K27ac, and SNF2H in human fusion positive myxoid liposarcoma (DL221 and MLS402) and human fusion negative liposarcoma cell lines (SW872). RNA-seq on DL221, MLS402 and SW872.