Sequencing facilitates quantitative analysis of transcriptomes in wild type and Lrp6-knockdown cells

Results and conclusions: We detected a substantial number of Lrp6 loss-induced AS events, and up to nearly 50 of them were exon-skipped. A gene ontology analysis of the conserved LRP6-driven network showed enrichment for ninety-four genes encoding proteins mainly involved in cellular and metabolic processes . Among them, a decade of genes with LRP6-dependent isoform expression showed identical splicing patterns on the exon level across species. The RNA sequencing results were further verified by Semiquantitative PCR. We conclude that LRP6 could regulate mRNA splicing.结果与结论：我们检测到大量由Lrp6缺失诱导的可变剪接（alternative splicing, AS）事件，其中近50个为外显子跳跃事件。对保守的LRP6调控网络开展基因本体（Gene Ontology, GO）富集分析，结果显示该网络富集得到94个编码蛋白质的基因，其功能主要涉及细胞过程与代谢过程。其中，十余个受LRP6调控异构体表达的基因，在跨物种的外显子层面展现出完全一致的剪接模式。本研究通过半定量聚合酶链式反应（semiquantitative PCR）进一步验证了RNA测序结果。综上，我们得出结论：LRP6可调控mRNA剪接过程。