Cxxc finger protein 1 maintains homeostasis and function of intestinal group 3 innate lymphoid cells with aging

To investigate the role of aging in the regulation of ILC3 development and function, we used aged mice and carried out single-cell RNA sequencing (BD Rhapsody) on aged gut ILC3s. ILC3s from 18-months-old male C57BL/6 mice were isolated from the small intestine and analyzed by single-cell RNA sequencing technology (BD Rhapsody).为探究衰老在固有淋巴细胞3型（Innate Lymphoid Cell 3, ILC3）发育与功能调控中的作用，本研究采用衰老小鼠模型，对其肠道ILC3开展单细胞RNA测序（BD Rhapsody）。具体实验中，我们从18月龄雄性C57BL/6小鼠的小肠中分离得到ILC3，并通过BD Rhapsody平台完成单细胞RNA测序分析。