Loss of O-glycans in HeV G affect HeV F incorporation and processing in pseudotyped virions, affecting viral entry.

A) Western blot of HeV/VSV viral lysates. HeV G (top panel) and HeV F (bottom panel) were detected as described in Fig 2. B) F incorporation and F processing of the HeV mutant pseudotyped virions determined by densitometry. F incorporation values were normalized to those of wt HeV. F processing was measured by the ratio of F1/ (F0+F1) for HeV pseudotyped virions. N = 3. Statistically significant differences, as determined by a Student’s t-test, are marked with an * (p<0.01). C) Viral entry of HeV/VSV virions at log dilutions of viral input. Values for the mutant virions are compared to wt HeV values. Virions expressing only HeV G (no HeV F) were used as a negative control. Averages and standard deviations are shown. N = 3.