Supplementary Material not marked-up

The supplementary material includes additional tables and figures to support the findings presented in the manuscript. Tables S.1-S.3 provide approximate values for the mean diameters and areas of individual PDAC cells, as well as median and p90 values of spheroid area (µm²) over time under various culture conditions. Figures S.1-S.5 illustrate the evolution of spheroid growth area over a 10-day period in collagen-based microfluidic devices, the quantification of permeability in type I collagen hydrogels through Darcy's Law, a visualisation of the search process to achieve a single connected component in the analysis of cellular nuclei, the viability of the PDAC spheroids in the high-collagen hydrogel (6 mg/ml) within the microfluidic device and cell distribution analysis after the hydrogel polymerization, respectively. The supplementary methods section showed the protocols followed for the experimental work of the figures presents in the Supplementary material.