Raw data for article "Effect of truffle-associated bacteria and fungi on the mycorrhi-zation of <i>Quercus ilex</i> seedlings with <i>Tuber melanosporum</i>"

The success of truffle cultivation depends especially on the quality of mycorrhizal seedlings, which are typically produced under aseptic conditions to avoid contamination by unwanted fungi. However, such practices may also eliminate beneficial microorganisms that could support truffle symbiosis, improving seedling quality and enhancing field establishment of truffle plantations. In this study, a set of 12 endophytic bacteria and fungi isolated from the <i>Tuber melanosporum</i> environment (gleba tissue, mycorrhizae and truffle <i>brûlé</i>) was tested on <i>Quercus ilex</i> seedlings under nursery conditions. Co-inoculation with a strain of <i>Agrobacterium tumefaciens</i> significantly enhanced root colonization by <i>T. melanosporum</i> and exhibited in vitro plant growth-promoting traits, supporting its potential role as mycorrhizal helper bacterium. In contrast, a strain of <i>Trichoderma harzianum</i> negatively affected mycorrhization. None of the tested strains showed significant effects on seedling growth. Our findings support the hypothesis that specific bacterial strains associated with truffles can act as mycorrhizal helper bacteria, highlighting their potential for co-inoculation strategies to enhance quality of truffle seedlings in nurseries. However, most of the tested microorganisms did not show a significant effect on seedling mycorrhization or growth, underscoring the need for further research to better understand the interactions within the mycorrhizosphere, in order to improve seedling quality.

块菌栽培的成效尤其取决于菌根幼苗的质量，这类幼苗通常在无菌条件下培育，以规避非目标真菌的污染。然而，此类培育方式或会同时清除那些能够助力块菌共生、提升幼苗品质并促进块菌人工林田间定植的有益微生物。本研究中，研究人员从黑孢块菌（Tuber melanosporum）生境（包括产孢组织、菌根以及块菌烧圈（brûlé））中分离得到12株内生细菌与真菌，并在育苗圃条件下对冬青栎（Quercus ilex）幼苗开展了接种试验。与根癌农杆菌（Agrobacterium tumefaciens）菌株复合接种，可显著提升黑孢块菌对幼苗根系的定殖效率，且该菌株在体外表现出促植物生长性状，证实其具备作为菌根辅助细菌（mycorrhizal helper bacterium）的应用潜力。与之相反，哈茨木霉（Trichoderma harzianum）菌株则会对菌根形成产生负面影响。所有受试菌株均未对幼苗生长产生显著影响。本研究结果支持"与块菌关联的特定细菌菌株可充当菌根辅助细菌"这一假说，凸显了复合接种策略在提升苗圃块菌幼苗质量方面的应用潜力。不过，多数受试微生物并未对幼苗菌根形成或生长产生显著作用，这表明亟需开展更多研究以深入解析菌根际内的互作机制，进而优化幼苗质量。

搜集汇总

背景与挑战

背景概述

该数据集提供了关于松露相关微生物对Quercus ilex幼苗与Tuber melanosporum菌根化影响的原始研究数据。研究发现，特定细菌如Agrobacterium tumefaciens能促进菌根化，而真菌如Trichoderma harzianum则有抑制作用，多数测试微生物无显著效果，支持了菌根辅助细菌的假设并突出了其在育苗中的潜在应用。