Growth and Survival Defects in <i>slik</i> Mutant Clones

(A) Wing imaginal disc with several homozygous slik1 mutant clones and homozygous wild-type twin clones (part of a leg disc is visible at upper right). The homozygous wild-type and mutant cells are produced in the same cell division, so differences in size reflect differences in growth or cell survival after clone induction. Homozygous slik1 mutant cells lack the βGAL marker protein and are unlabeled (black). Homozygous wild-type cells have two copies of the marker and appear brighter than heterozygous slik1/+ cells. (B) Area measurements of 48 pairs of homozygous slik1 mutant and wild-type twin clones. (C and D) Wing disc with a large homozygous Minute+ slik1 mutant clone produced in a Minute heterozygous background. Slik protein is shown in red. Blue shows DAPI-labeled nuclei. (C) and (D) are different optical sections of the same disc. (D) shows the pyknotic nuclei below the epithelial layer. (E and F) Wing discs with large homozygous Minute+ slik1 mutant clones. Red shows a single optical section showing Slik protein. Green shows a projection of several optical sections showing TUNEL labeling to visualize apoptotic cells. (F) Mid-third instar disc. (G) DAPI and TUNEL labeling of a slik1 homozygous mutant wing disc.