RNA-sequencing on Nrn1-/- and Nrn1+/- OTII cells recovered from OVA-peptide induced anergy TCRa-/- host mice. RNA-sequencing on Nrn1-/- and Nrn1+/- OTII cells recovered from OVA-peptide induced anergy TCRa-/- host mice

Nrn1-/- and Nrn1+/- Thy1.1+ OTII cells were cotransferred with wt Treg cell into TCRa-/- hosts and subjected to soluble OVA peptide i.v. treatment for anergy induction on day 1, 4, and 7 post transfer. Nrn1-/- and Nrn1+/- Thy1.1+ OTII cells were recoverred by cell sorting 13 days after cell transferand subjected to RNA-Sequencing analysis Overall design: Expression profiling by high throughput sequencing performed on Illumina HiSeq V2 Systems 75 cycles (1x75bp).