The goal of this study is to develop a novel method for reducing unwanted genomic editing by CRISPR/Cas9 and improve specificity in a variety of cellular contexts.

NIAID Data Ecosystem40

SpCas9 with 17-bp R-loop containing 2 terminal mismatches (State II - precleavage)

CRISPR-Cas9结构机制

基因编辑特异性

SpCas9 with 17-bp R-loop containing 2 terminal mismatches (State II - precleavage)

NIAID Data Ecosystem30

CRISPR GUARD: short guide RNAs protect off-target sites from Cas9 nuclease activity

CRISPR脱靶保护

基因编辑特异性

Precise genome editing using CRISPR-Cas9 driven nucleases and base editors are promising therapeutic avenues for genetic diseases, although collateral damage caused by off-targets is a significant saf

NIAID Data Ecosystem40

Genome-wide Profiling of Adenine Base Editor Specificity by EndoV-seq. Homo sapiens

碱基编辑器脱靶检测

基因编辑特异性

EndoV-seq utilizes Endonuclease V (EndoV) to nick the inosine (I)-containing DNA strand of genomic DNA that has been deaminated by ABE in vitro. The treated DNA is then whole-genome sequenced to ident

NIAID Data Ecosystem30

sgRNA library with comprehensive mismatch

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