Representative data showing the effect of rivaroxaban on platelet aggregation induced by collagen.

PRP was pretreated with various doses of rivaroxaban for 15 min, and then stimulated by 1.0 μg/ml collagen or vehicle for 5min. The reaction was terminated by the addition of ice-cold EDTA (10 mM) solution. Platelet aggregation was detected by an aggregometer with laser scattering system. The black line indicates the percentage of transmittance of each sample (PRP recorded as 0%, and PPP was recorded as 100%). The blue line indicates small aggregates (9–25 μm); the green line, medium aggregates (25–50 μm); the red line, large aggregates (50–70 μm). The distributions (%) of aggregated particle size were measured by AUC of each particle size. Representative results obtained from ten healthy donors are presented.