Study of bone marrow Nestin+ mesenchymal stromal cells in control and leukemic mice

NRAS-G12D+ mice show reduced numbers of mesenchymal stromal cells together with increased apoptotic rates as compared to healthy controls. Male Nes-gfp mice were lethally irradiated and transplanted with bone marrow cells from previously pI-pC induced control (Mx1-Cre-negative NRAS-G12D-positive) or leukemic (Mx1-Cre-positive NRAS-G12D-positive) mice, at the age of 28 weeks. Bone marrow Nes-gfp-positive mesenchymal stromal cells were FACS-sorted 4 weeks after the transplant, and total RNA was isolated and used for RNA sequencing.