Western blot screening of the 20 drugs and optimization at different times in JHU029, HTB-43 and CCL-138 (parental and resistant) cell lines. Treatment was tested at 8, 24 and 48 hours and the drug 13
Protein lysates of Xrn1 knock out (ko) A549 cells infected with WT PR8 or PR8-PA(∆X), or mock infected, were probed with antibodies against PR8 PA or beta-tubulin as a loading control.
