Bioinformatic analysis of isolated NS1 ED helix-helix dimer characteristics.

ED helix-helix dimers identified as PDB ID_chains (e.g. dimer 3O9U_AC is formed by chains A and C of structure 3O9U). The helix-helix dimer formed by chains A and B of PR8 ED structure 2GX9 is identified by a superscript HH to distinguish it from the strand-strand dimer also made by those chains (see Table 4). The ED dimer formed by chain A of the full-length VN/04 NS1 structure 3F5T is distinguished from the RBD dimer formed by the same chain (see Table 5) by a superscript ED. All values calculated using either ProtorP (*) or ProFace (**). Symmetric dimer 3O9T_AA (§) is excluded from calculation of mean and standard deviation due to the presence of a molecule of PEG2000 at the helix-helix dimer interface. To facilitate fair comparison, the VN/04 NS1 ED symmetric dimer 3F5T_AAED(†) was not included in the calculations as it is part of a full-length NS1 structure.